Sulfotransferase gene super family and substrate specificity
نویسندگان
چکیده
منابع مشابه
Substrate specificity and safener inducibility of the plant UDP‐glucose‐dependent family 1 glycosyltransferase super‐family
Plants contain large numbers of family 1 UDP-glucose-dependent glycosyltransferases (UGTs), including members that conjugate xenobiotics. Arabidopsis contains 107 UGT genes with 99 family members successfully expressed as glutathione transferase (GST)-fusion proteins in E. coli. A high-throughput catalytic screen was developed based on quantification of the fusion by measuring GST activity. UGT...
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We identified a cDNA encoding a putative cytosolic sulfotransferase (SULT) by searching the expressed sequence tag database of Bombyx mori, and subsequently obtained the full-length cDNA for this gene via rapid amplification of cDNA ends (RACE). We designated this gene bmST1, and showed by sequence analysis that it belongs to a novel SULT family. The tissue specificity of bmST1 mRNA expression ...
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Spodoptera frugiperda retinol dehydratase catalyzes the conversion of retinol to the retro-retinoid anhydroretinol. It shares sequence homology with the family of mammalian cytosolic sulfotransferases and provides the first link between sulfotransferases and retinol metabolism. In this study the enzymatic properties of retinol dehydratase were examined using bacterially expressed protein. We sh...
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The functions of SWEET (Sugar Will Eventually be Exported Transporter) proteins have been studied in a number of crops, but little is known about their roles in cucumber (Cucumis sativus L.), a model plant for studying stachyose metabolism and phloem function. Here, we identified 17 cucumber SWEET genes (CsSWEETs), located on chromosomes 1-6, and classified them into four clades. Two genes from...
متن کاملMolecular mechanism of substrate specificity for heparan sulfate 2-O-sulfotransferase.
Heparan sulfate (HS) is an abundant polysaccharide in the animal kingdom with essential physiological functions. HS is composed of sulfated saccharides that are biosynthesized through a complex pathway involving multiple enzymes. In vivo regulation of this process remains unclear. HS 2-O-sulfotransferase (2OST) is a key enzyme in this pathway. Here, we report the crystal structure of the ternar...
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ژورنال
عنوان ژورنال: Drug Metabolism and Pharmacokinetics
سال: 1998
ISSN: 0916-1139
DOI: 10.2133/dmpk.13.supplement_88